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Series GSE79488 Query DataSets for GSE79488
Status Public on Jan 01, 2019
Title Transcriptomic analysis of gene expression in adult Drosophila hemocytes
Organism Drosophila melanogaster
Experiment type Expression profiling by high throughput sequencing
Summary Hemocytes are phagocytic blood cells that act as the first line of defense against bacterial pathogens in the fruit fly, Drosophila melanogaster. To gain insight into the immune-regluated transcriptional response in this cell type, we sequenced uninfected, mock (PBS) infected, and Staphylococcus aureus infected hemocytes collected from adult flies. Wildtype and A2bp1 RNAi hemocytes expressing a mouse-CD8-GFP fusion protein were isolated using immunselection and sequenced using 50bp single end reads on an Illumina HiSeq platform. A2bp1 RNAi hemocytes express the TRiP short hairpin RNA HMS00478 (which targets all isoforms of Ataxin-2 binding protein 1) under the hemolectinΔGAL4 promoter.
 
Overall design Examination of gene expression profiles in phagocytic blood cells from adult fruit flies. 18 RNA samples were analyzed by RNAseq (3 replicates x 3 treatments x 2 genotypes). Treatments were uninfected, mock-infected and S. aureus-infected. Genotypes were wild-type and RNAi vs. A2BP1.
 
Contributor(s) Nazario-Toole A, Wu L, Mount SM
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Submission date Mar 22, 2016
Last update date May 15, 2019
Contact name Stephen M. Mount
E-mail(s) smount@umd.edu
Phone 3014056934
Organization name Univ. Maryland
Department Cell Biol. & Mol. Genetics
Street address HJP, 4065 Campus Dr.
City College Park
State/province MD
ZIP/Postal code 20742
Country USA
 
Platforms (1)
GPL19951 Illumina HiSeq 1500 (Drosophila melanogaster)
Samples (18)
GSM2095899 Attp2_Uninjected_Set1
GSM2095900 HMS00478_Uninjected_Set1
GSM2095901 Attp2_Saureus_Set1
Relations
BioProject PRJNA315975
SRA SRP072173

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE79488_A2bp1_log-normalized_count.csv.gz 1.2 Mb (ftp)(http) CSV
GSE79488_A2bp1_original_count.csv.gz 389.6 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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